The Mechanism of Frabot Test for Uric Acid

Uric acid is an Analyte. Excess uric ─ hyperuricemia leads to gout and renal stones. Frabot noted that added alkaline tungstate solution developed intense blue colour. A colorimetric method widely utilized quantify depends on the reduction of chromogen sodium by produce a measurable colour change. The reaction route from initial products coloured compounds oxido-degradation organic substance has not been advanced. In this communication electron flow given in each step series reactions take place. These are isomerization imidol, Michael addition enone, oxirane formation redox step, hydrolysis imido group, ring opening, contraction five- member ring, opening via alkoxide, finally assisted decarboxylation end product, 5-ureido hydantoin (allantoin).